| 초록 |
Recently, several techniques have been used to manufacture porous polymeric scaffolds having 3-dimensional structure. But these scaffolds usually have limitations for some applications because of their hydrophobicity and stiffness. These properties led to inefficient cell seeding and growth as well as handling difficulties. In our previous study, porous bioartificial scaffolds composed of alginate (natural polymer; hydrophilic, biocompatible, brittle) and polyvinyl alcohol (PVA) (synthetic polymer; hydrophilic, flexible) with different alginate and PVA compositions were fabricated by a modified freeze-drying method and the following chemical crosslinking of sodium alginate and PVA in the hybrid scaffolds. It was observed that the pore size of the scaffolds could be controlled by adjusting alginate and PVA ratio. In this study, we fabricated the alginate/PVA scaffolds by a modified freeze-drying method and the following physical crosslinking of PVA (freeze-thawing method) to reduce potential cytotoxicity of residual glutaraldehyde which is used as a PVA crosslinking agent. The alginate/PVA hybrid scaffolds having a different alginate and PVA compositions were investigated by compressional mechanical test, water absorption, dimensional stability, and in vitro cell culture (chondrocyte).
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