| 초록 |
Recently, the bone regeneration using tissue engineering technique and local gene delivery system have been considered as one of the promising therapeutic methods. In this study, we prepared plasmid DNA (with BMP-2 encoding)-loaded polycaprolactone (PCL)/Pluronic F127 membrane to enhance the osteogenic differentiation of adipose stem cells (ASCs). For effective plasmid DNA release from the membrane and the following transfection into the cells of plasmid DNA, the PCL membrane was hydrophilized by Pluronic F127 and the plasmid DNA was complexed with polyethyleneimine-polyethylene glycol (PEI-PEG). The pDNA/PEI-PEG complex was continuously released from the PCL/Pluronic F127 membrane for a long period of time (more than 3 months). From the in vitro transfection assays, it was observed that the pDNA/PEI-PEG released from the membrane was efficiently transfected into the ASCs, inducing the osteogenesis effectively. |
