| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 봄 (04/12 ~ 04/13, 제주 ICC) |
| 권호 |
32권 1호 |
| 발표분야 |
나노바이오기술용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Efficient Gene Delivery by PEGylated Adenovirus using Specific Cell-targeting Ligand and Avidin-Biotin Interaction |
| 초록 |
Gene delivery systems using adenovirus vector exhibit high transduction efficiency and gene expression. In this study, the adenovirus (Ad) expressing the green fluorescent protein (GFP) was modified by polyethylene glycol (PEG) to reduce toxicity and immunogenicity. In addition, epidermal growth factor (EGF) was used as specific cell-targeting ligand to overcome problematic nonspecific transduction. Pegylated EGF was attached on the surface of adenovirus using avidin-biotin interaction. We confirmed that Ad-PEG-EGF complexes increased GFP expression level compared to Ad-PEG in EGF receptor positive cell line (A431 cell), but not in EGF receptor negative cell line (MCF-7 cell). Thus, the adenovirus immobilized with PEG-EGF based on biotin-avidin interaction enhanced both stability from nonspecific immune system and transfection efficiency on the specific cell line. |
| 저자 |
박지원, 목혜정, 박태관
|
| 소속 |
한국과학기술원 |
| 키워드 |
Adenovirus; EGF; PEG; avidin-biotin interaction
|
| E-Mail |
|
