| 학회 |
한국화학공학회 |
| 학술대회 |
2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 |
15권 1호, p.406 |
| 발표분야 |
생물화공 |
| 제목 |
Enhancing organic solvent stability of Candida antarctica lipase B in hydrophilic solvents |
| 초록 |
The production of biodiesel by enzymatic transesterification has attracted much attention for high purity product and enables easy separation. Candida antarctica lipase B is one of the most important enzyme in the biodiesel reaction. In reaction, CALB shows low organic solvent stability while having relation with methanol.In water-miscible solvents, water molecule seems to play an important role in biological structure and function. Water molecules in the enzyme are stripped away or replaced with hydrophilic solvent molecules and this causes deformation and denaturation of the enzyme.Increase of hydrogen bond interaction can make water molecule less strips off and more stable in hydrophilic organic solvents. Hydrogen bond interaction can be increased by shorten the distance of amino acids and water molecule and also increase electrostatic interaction. ASP and ASN was replaced to GLU and GLN of CALB for in silico mutagenesis. In vitro experimental data will be displayed. |
| 저자 |
박현준1, 박경문2, 유영제3
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| 소속 |
1Graduate Program in Bioengineering, 2Seoul National Univ., 3Department of Chemical System Engineering |
| 키워드 |
organic solvent; Candida antarctica lipase B;
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| E-Mail |
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| 원문파일 |
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