| 초록 |
Recently, natural polymers are frequently comingled with synthetic polymers either by physical or chemical modification to prepare numerous tissue engineered graft with promising biological function, strength and stability. Aim of this research is to fabricate bilayered tubular scaffold as tissue engineered vascular grafts (TEVGs). In this study a novel double layered polycaprolactone-polyethylene glycol methyl ether (PCL-PEGME) loaded with Vascular Endothelial Growth Factor (VEGF) as inner layer and PCL/Chitosan loaded with Platelet Derived Growth Factor (PDGF) as outer layer tubular scaffold was prepared by the Electrospinning technique. Nano/microfibrous mats prepared from electro-spinning were characterized for fiber diameter, porosity, wettability and mechanical strength. The inner layer with VEGF biomolecule provides biocompatible scaffolds having desired cellular response. For sustain release of PDGF, cross linked PCL /Chitosan is used to so that the ultimate graft may be more stable without thrombosis and burst. Biological efficacy on both biodegradable scaffolds was assessed by rat bone marrow mesenchymal stem cells, and PCL-PEGME showed feasibility for use as an inner layer by inducing endothelial-specific gene expression and PCL-Chitosan as an outer layer on dual-layered without sacrificing tensile strength, small-diameter blood vessels. Therefore, scaffolds fabricated from this research could be potential sources for tissue engineered vascular graft and could also overcome the well-known drawbacks, such as thrombogenicity and stenosis, in managing vascular disease. |
