| 학회 |
한국공업화학회 |
| 학술대회 |
2006년 봄 (05/12 ~ 05/13, 전북대학교) |
| 권호 |
10권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Expression and characterization of 3-ketoreductase involved in biosynthesis of Sch-47554 from Streptomyces sp SCC-2136 |
| 초록 |
Sch-47554 and Sch-47555 is an antifungal, antibacterial and enzyme inhibitor angucycline polyketide produced by S. sp. SCC-2136. Sequence analysis of Sch-47554 and Sch-47555 biosynthesis gene cluster revealed the presence of 55 open reading frames involved in biosynthesis. SchS-1 in the gene cluster was identified as 3-ketoreductase by amino acid sequence comparison with other genes of the same family from other several sources. The gene encoding 341 amino acids was amplified, sequenced and expressed. The deduced amino acid sequence of SchS-1 shows 65% similarity to LanT from S. cyanogenus and 66% to UrdT from S. fradiae. The expressed protein was characterized by enzyme assay. |
| 저자 |
Pokhrel Trilochan, 송재경, 류광경, Devi Bahadur Basnet, 윤준식
|
| 소속 |
선문대 |
| 키워드 |
Ketoreductase; Streptomyces sp.SCC-2136; Sch-47554; Sch-47555
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| E-Mail |
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