| 학회 | 한국공업화학회 |
| 학술대회 | 2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 23권 2호 |
| 발표분야 | 포스터_생물공학 |
| 제목 | Mutation in M13 phage for mass production of ssDNA for nano material |
| 초록 | M13 phage is a filamentous phage that infects E.coli though F pilus. When M13 phage enters in E.coli, it replicates itself using rolling circle replication. In this special replication, p5(pV) acts as a regulator. In stage 2, production of single strand DNA follows Fibonacci increase. And in stage 3 it follows arithmetic increase on the assumption that replication rate is constant. For the single strand production, longer stage 2 is favorable. In this rolling circle replication, p5 concentration is a branch point between stage 2 and stage 3. If accumulation of p5 is fast, shift to stage 3 occurs fast. However, accumulation of p5 is slow, shift to stage 3 occurs lately. It means that we can get longer stage2. Accumulation of p5 can be controlled in translational level. Ribosome binding affinity is affected by RBS sequence, spacer sequence and coding sequence. If spacer sequence is changed, ribosome binding affinity is changed and protein expression level is changed. |
| 저자 | 이보영, 오민규 |
| 소속 | 고려대 |
| 키워드 | M13 phage; plasmid replication; ssDNA |
