| 학회 | 한국화학공학회 |
| 학술대회 | 2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터) |
| 권호 | 25권 1호, p.371 |
| 발표분야 | 생물화공(Biochemical Engineering) |
| 제목 | Fluorescence assay for RNase H utilizing target-triggered DNA polymerase activity |
| 초록 | We herein developed a novel and sensitive method to analyze RNase H activity utilizing DNA polymerase activity, triggered by the RNase H catalyzed hydrolysis of RNA strand in DNA:RNA hybrid. In this study, the key component, a detection probe, was rationally designed by combining a DNA polymerase-specific aptamer and a RNase H substrate. RNase H degrades the RNase H substrate and releases it from the detection probe [1]. Thus, the polymerase activity is recovered and initiates primer extension reaction coupled with a TaqMan probe [2]. Based on this method, we successfully determined the RNase H activity. Since this method consisted of separated target recognition step and signal transduction step, this new strategy could be further applied to the development of universal enzyme activity assays by rationally designing of detection probes. References 1. A. Muroya, D. Tsuchiya, M. Ishikawa, M. Haruki, M. Morikawa, S. Kanaya, and K. Morikawa, Protein Sci., 10, 707-714 (2001). 2. K. S. Park, C. Y. Lee, and H. G. Park, Chem. Commun., 51, 9942-9945 (2015). |
| 저자 | 정유진1, 이창열1, 박기수2, 박현규1 |
| 소속 | 1KAIST, 2건국대 |
| 키워드 | 생물화학공학 |
| 원문파일 | 초록 보기 |
