| 초록 |
Alginate, the most abundant sugar in brown algae, is a potential biochemical feedstock for biofuel production. However, alginate is a difficult-to-ferment sugar that cannot be used for biofuel production by industrial microbes. This problem can be overcome through the development of metabolically engineered microbe to efficiently utilize the alginate. The alginate derived monomer is non-enzymatically converted to 4-deoxy-L-erythro-hexoseulose uronic acid (DEH), then reduced to 2-keto-3-deoxy-D-gluconate by a DEH reductase, and metabolized through the Entner-Doudoroff pathway. These metabolites can be converted to bioethanol in industrial microbes. Among the alginate metabolic enzymes, DEH reductase is the key enzyme. In this study, we identified and characterized the NADH/NADPH dependent DEH reductase from Vibrio splendidus. The metabolically engineered E. coli was developed by transforming the DEH reductase gene and could produce bioethanol from alginate saccharification solution. |
