| 초록 |
Dimethylsulfoxide (DMSO) was used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells and tissues despite its cytotoxicity and effects on differentiation. Here, we report that polyampholytes (COOH-PLLs) with an appropriate ratio of amino and carboxyl groups show higher cryopreservation efficiency and lower cytotoxicity than DMSO. We propose that cryoprotection of COOH-PLLs is exerted by protecting cells from stresses such as drastic changes in soluble space size and osmotic pressure during freezing. We also show the development of an effective vitrification method that does not impair either the macro- or microstructures of cell sheets, and thereby possesses significant potential for applications related to clinical cell sheet therapy. Thus, these polyampholytes can replace DMSO as new materials for CPA in various preserving functions and will be useful in studies elucidating the mechanisms of cryopreservation. |
