| 초록 |
Rapid re-endothelialization at the inserted stent in atherosclerotic lesion may be effective for reducing or preventing local restenosis and thrombosis. In this study, fibroblast-derived extracellular matrix (FDM) was coated on cobalt-chromium alloy (Co-Cr), resulting in a reservoir for the delivery of late endothelial progenitor cell (late EPC). Since late EPC can differentiate into endothelial cell (EC) in a suitable condition such as extracellular matrix (ECM), we established a covalent bond formation between Co-Cr and FDM, using carboxyl ended-self assembled monolayer (SAM) of phosphonic acid on Co-Cr surface. We assumed that covalently bound FDM on Co-Cr is effective for late EPC delivery even in the blood flow. Fibroblasts were decellularized, and the FDM structure was defined by immunostaining. In conclusion, we are convinced that FDM-covered Co-Cr stent surface may accelerate re-endothelialization as compared to bare metal surface. |
