| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1534 |
| 발표분야 |
생물화공 |
| 제목 |
Prediction of metabolic fluxes based on genomic context and flux-converging pattern analyses for genome-scale simulation |
| 초록 |
Constraints-based flux analysis including flux balance analysis (FBA) has found numerous applications in the field of systems biology and biotechnology. However, the FBA simulation without additional information has some limitations such as inaccurate prediction of fluxes and existence of multiple solutions for an optimal objective value. Here, we report a strategybased on FBA combined with grouping reaction constraints that restrict the achievable flux ranges of grouped reactions by genomic context and flux-converging pattern analyses. FBA of Escherichia coli genome-scale metabolic model wasperformed to validate the strategy under different genotypic and environmental conditions. The predictionswith the constraintsagreed wellwith experimentally measured fluxes. [This work was supported by the Korean Systems Biology Research Project (20100002164) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea. Further support by the World Class University Program (R32-2009-000-10142-0) through the National Research Foundation of Korea funded by the MEST is appreciated.] |
| 저자 |
박종명, 김태용, 이상엽
|
| 소속 |
한국과학기술원 |
| 키워드 |
Flux balance analysis; grouping reaction constraints; genomic context analysis; flux-converging pattern analysis
|
| E-Mail |
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| 원문파일 |
초록 보기 |
