| 초록 |
The development of an efficient single nanoparticle–based plasmonic clinical sensor for direct recognition of protein hot–spot mutations in human breast tumor surgical specimens is presented. DNA–protein interactions are kinetically analyzed on the clinical sensor surface via simple measurement of the localized surface plasmon resonance Δλmax. Accordingly, the hot–spot mutant proteins are 13.86–fold higher in the dissociation constant values and 29.59–fold lower in the relative transcriptional activities than those of the normal proteins. The clinical validity of the sensor is also confirmed by identifying the importance of the mutated site of the protein with high specificity, ultra–sensitivity, and accuracy in comparison with conventional analytical methods. The present novel clinical sensor demonstrates strong potential as a promising system for prognosis and monitoring treatment responses in a variety of cancers. |
