| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 봄 (04/27 ~ 04/29, 부산 BEXCO) |
| 권호 |
22권 1호, p.792 |
| 발표분야 |
이동현상 |
| 제목 |
컬러-코딩된 하이드로젤 마이크로입자를 이용한 히스톤 변형의 다중검출 |
| 초록 |
The epigenetic modifications in gene expression are influenced by environment. The individualized genome-wide analysis has become a essential technology for recent trends in clinical field. We report 3-multiplex detection of changes in modified histones using Quantum Dot-encoded polyethylene glycol diacrylate hydrogel microparticles. We present the simultaneous detection of acetylation of lysine 9 of histone 3, di-methylation of H3K9, and tri-methylation of H3K9 from three distinct regions in the brain of cocaine-exposed mice. Our hydrogel-based epigenetic assay enabled relative quantification of the three histone variants from only 10 μL of each brain lysate per mouse. We proved that the cocaine treatment induced a significant increase of acetylation while a notable decrease in methylation in NAc. |
| 저자 |
염상윤1, 손충현2, 김병선3, 남은주3, 강해민3, 신효근3, 김소현3, 이현주3, 최정규1, 임혜인3, 조일주3, 최낙원3
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| 소속 |
1고려대, 2KIST, 3KIST 뇌과학(연) |
| 키워드 |
이동현상 |
| E-Mail |
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| 원문파일 |
초록 보기 |
