| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
유기반도체 재료 및 소자 |
| 제목 |
Molecular Design and D-A Distance Modulation for Conjugated Polyelectrolytes-based FRET DNA detection |
| 초록 |
Electrostatic interaction between cationic conjugated polyelectrolytes (CPs) and dye-labeled DNA can be coordinated to give rise to efficient fluorescence resonance energy transfer (FRET) for sequence-specific DNA assays. Same conjugated polyelectrolyte structures with/without molecular spacers are compared in functioning as a donor in FRET-based DNA detection. Fluorescence quenching of the acceptor via electron transfer (eT) in the electrostatic DNA-polymer complex is a main concern in improving the detection sensitivity. This study shows that thermodynamically favorable eT quenching can be controlled kinetically by modulating the D-A intermolecular distance using molecular spacers, which suggests an important molecular design guideline for efficient CPs-based FRET donors. In addition, several approaches to control D-A distance in subnanometer scale will be discussed for the optimized CPs-based FRET DNA detection. |
| 저자 |
우한영 |
| 소속 |
부산대 |
| 키워드 |
conjugated polyelectrolytes; FRET; biosensor
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| E-Mail |
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