| 학회 |
한국고분자학회 |
| 학술대회 |
2012년 봄 (04/12 ~ 04/13, 대전컨벤션센터) |
| 권호 |
37권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Highly sensitive and selective potassium ion detection based on cationic conjugated polyelectrolytes |
| 초록 |
We demonstrate highly sensitive and selective potassium ion detection against excess sodium ions in water, by modulating the interaction between the G-quadruplex-forming molecular beacon aptamer (MBA) and cationic conjugated polyelectrolyte (CPE). The K+-specific aptamer sequence in MBA is used as the molecular recognition element and the high binding specificity of MBA for potassium ions offers selectivity against a range of metal ions.Conformational changes of MBA as well as fluorescence (of the fluorophore in MBA) quenching or amplification via fluorescence resonance energy transfer from CPEs provide clear signal turn-off and –on in the presence or ab-sence of K+. The detection limit of the K+ assays is determined to be ~ 1.5 nM in the presence of 100 mM Na+ ions, which is ~3 orders of magnitude lower than those reported previously. |
| 저자 |
김보람, 우한영
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| 소속 |
부산대 |
| 키워드 |
conjugated polyelectrolytes; FRET; potassium ion detection
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| E-Mail |
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