| 초록 |
Delivery of small interfering RNA (siRNA) has shown great potential for treatment of various diseases, including infectious disease. However, naked siRNA should overcome substantial challenge for in vivo applications due to its limited stability in the serum. Thus suitable delivery carriers are required for successful and efficient siRNA delivery. We hypothesized that chitosan modified with positively charged oligopeptides could be useful to enhance the stability chitosan/siRNA complexes and to improve the transfection efficiency. Various physicochemical properties of chitosan derivative/siRNA complexes, including particle size, surface charge, capacity of complexation, cytotoxicity, and in vitro transfection efficiency, were investigated. We also tested in vivo therapeutic efficacy of chitosan derivative/siRNA nanoparticles using a mouse model. This approach to developing a novel delivery system using natural polymers may provide a useful means to many gene delivery applications. |
