| 학회 | 한국화학공학회 |
| 학술대회 | 2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 | 10권 1호, p.468 |
| 발표분야 | 생물화공 |
| 제목 | Manufacturing of collagen membrane for bioartificial skin |
| 초록 | Human keratinocytes can be growen reliably and reproducibility in vitro to form multilayered epithelium. These sheets of cultured keratinocytes have been used to patients with severe burns and leg ulcers. There is a delay of 2 to 3 weeks for culture of the sheets of keratinocytes. So we made an artificial skin comprised of a keratinocyte in skin and a type I collagen membrane. In this work, we made a collagen membrane for the purpose of scaffold for artificial skin. And we showed primary culture keratinocyte, and cultured on the collagen membrane. The collage membrane could be obtained using 5 mg/ml collagen solution and lyopilization and then dry methods. To develop the artificial skin 500,000 cells/cm2 of keratinocyte were cultured on the collagen membrane. Consequentely, it is possible that to make the bioartificial skin within 10 days. |
| 저자 | 서영권1, 박정극1, 송계용2, 서성준3, 김영진4 |
| 소속 | 1동국대 화학공학과, 2중앙대 병리학과, 3중앙대 피부과, 4(주) 라이프코드 |
| 키워드 | collagen membrane; artificial skin |
| 원문파일 | 초록 보기 |
