| 초록 |
Effects of chemical or biological compounds on mammalian pigmantation have been reported by many researcher. Many melanocyte or artificial skin models have been used to evaluate the potential efficacy of melanogenic compounds to regulate pigmentation. However, in the skin and hair, melanocytes interaction with keratinocyte, fibroblasts, and other cell types, and testing of compounds on melanocytes alone in culture does not allow one to observe the interactions with those other cell types, such as wound occur in vivo. We have developed a model that use immotalized murin melanocytes(melana) or melanocyte in pure / coculture. We developed a melana or mlanocyte-human skin keratinocyte pure / coculture protocol that allows testing of compounds for potential effects on pigmentation. We have standardized it with known melanogenic inhibitor (arbutin) and stimulator (α-melanocyte stimulating hormone, UVB-irridation). After treatment of melanocytes with bioactive compounts, cell viability, total melanin contents, and tyrosinase activity are measured. |
