| 초록 |
Biodiesel is commercially produced through chemical methods with acid or base catalysts. As an alternative to these chemical methods, enzymatic biodiesel production has been extensively studied because it has several advantages such as much lower energy requirement and less wastewater discharge compared with chemical methods. However, enzyme is very expensive and easily deactivated by methanol. Therefore, the reuse and protection of the enzyme from methanol inhibition are crucial for commercial enzymatic biodiesel production. In this study, Pseudo-Two Phase Bioreactor, a modified version of conventional two-phase bioreactor, is newly suggested to avoid methanol inhibition. In the P-TPB, an additional organic solvent as a second phase was added to biodiesel production to minimize the direct contact between enzyme and undissolved methanol. Through this approach, biodiesel was produced with satisfactory conversion. |
