화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2008년 가을 (10/09 ~ 10/10, 일산킨텍스)
권호 33권 2호
발표분야 의료용 고분자 부문위원회
제목 Galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine as a gene carrier for hepatocyte targeting
초록 In the present study a chitosan derivative, galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine (Gal-PEG-CHI-g-PEI), was investigated as a potential hepatocyte-targeting gene carrier. The composition of Gal-PEG-CHI-g-PEI was characterized using 1H nuclear magnetic resonance (1H NMR), and the particle size and zeta potential of Gal-PEG-CHI-g-PEI/DNA complexes were measured using dynamic light scattering (DLS). The Gal-PEG-CHI-g-PEI exhibited lower cytotoxicity compared to PEI 25K as a control. Likewise, Gal-PEG-CHI-g-PEI/DNA complexes showed good hepatocyte specificity. Furthermore, Gal-PEG-CHI-g-PEI/DNA complexes transfected liver cells more effectively than PEI 25K in vivo after intravenous (i.v.) administration. Together, these results suggest that Gal-PEG-CHI-g-PEI, which has improved transfection efficiency and hepatocyte specificity both in vitro and in vivo, may be useful for gene therapy.
저자 강호림, 김유경, 권정택, 조명행, 로히다스, 다난자이, 조종수
소속 서울대
키워드 gene therapy; targeting gene delivery; galactosylated poly(ethylene glycol)-chitosan-graft-polyethylenimine; cytotoxicity; transfection efficiency; intravenous administration
E-Mail chocs@plaza.snu.ac.kr