| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 |
14권 1호, p.494 |
| 발표분야 |
생물화공 |
| 제목 |
Multiple gene deletion of Mannheimia succiniciproducens using mutant lox sequences |
| 초록 |
A markerless gene deletion method was developed to generate genetically modified Mannheimia succiniciproducens, which is a rumen bacterium producing succinic acid as a major metabolite. Cre recombinase was transiently expressed using a temperature sensitive plasmid to excise the antibiotic marker between the mutant lox sites. As a demonstration of the method, ldhA gene and oadGAB operon was sequentially deleted without leaving an antibiotic marker inside the genome of M. succiniciproducens. |
| 저자 |
김지만1, 이상엽2
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| 소속 |
1Metabolic and Biomolecular Engineering National Research Laboratory, 2Department of Chemical & Biomolecular Engineering and BioProcess Engineering Research Center |
| 키워드 |
Mannheimia succiniciproducens; Temperature sensitive plasmid; gene deletion; mutant lox sequences
|
| E-Mail |
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| 원문파일 |
초록 보기 |
