| 학회 |
한국화학공학회 |
| 학술대회 |
2005년 가을 (10/21 ~ 10/22, 인하대학교) |
| 권호 |
11권 2호, p.2101 |
| 발표분야 |
생물화공 |
| 제목 |
Metabolic engineering of E. coli for the production of L-valine and its transcriptome/fluxome analysis |
| 초록 |
We constructed L-valine production stain with Escherichia coli W3110 by targeted genetic modification and identified the effect of the biosynthesis of L-valine on cell physiology by combined transcriptome and fluxome analysis. The L-valine-producing strain was constructed by releasing two regulatory mechanisms, feedback inhibition and attenuation. Two amino acids alterations were introduced into ilvH which is subject to feedback inhibition by site-directed mutagenesis. The leader region of ilvGMEDA and ilvBN operon which is involved in attenuation was changed with the strong tac promoter by homologous recombination. Further improvement of the L-valine-producing strain was achieved by knocking out leuA, panB and ilvA thus making more substrate available for the L-valine biosynthesis. Combined transcriptome and fluxome analysis reveals that an increased pyruvate and ketoisovalerate availability is essential to direct the flux into the L-valine biosynthesis. Furthermore, target genes for further metabolic engineering can be selected from the combined analysis data. (This work was supported by Korean Systems Biology Research Grant, M10309020000-03B5002-00000 from the MOST.) |
| 저자 |
박진환1, 이광호2, 김태용1, 이상엽1
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| 소속 |
1한국과학기술원, 2한국과학기술원 / CJ(주) |
| 키워드 |
L-valine; metabolic engineering; transcriptome; fluxome
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| E-Mail |
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| 원문파일 |
초록 보기 |
