| 초록 |
In this study, we developed a facile method to functionalize and stabilize the surface of graphene oxide (GO) with a cationic polymer, poly(2-dimethylaminoethyl methacrylate) (poly(DMAEMA)), using a biomimetic adhesive molecule, 3,4-dihydroxy-L-phenylalanine (dopa). The surface of GO can be readily modified with dopa-functionalized poly(DMAEMA). The resulting GO-dopa-poly(DMAEMA) interacted with siRNA to form multi-layered nano-structures, which provided excellent protection of siRNA form the attack of serum-driven nucleases. In addition, GO-dopa-poly(DMAEMA) demonstrated significantly improved cellular uptake and enhanced gene silencing efficiency, compared to unmodified GO and PEI 25k. Considering its low toxicity and desirable transfection efficiency, GO-dopa-poly(DMAEMA) would be potential candidate for siRNA delivery. |
