| 초록 |
We performed a study about stabilized plasmid-lipid nanoparticle. It was used to detergent dialysis method which is compose of particle using several ‘fusogenic’ lipid and detergent in aqueous solution. For formed stable micelle, we use DOPE, which is known to help particle formation and positive charge lipid was DOTAP which induce encapsulation of plasmid-DNA. We characterized block copolymers that consist of poly(ethylene glycol)-b-poly(lysine)-ss-poly(caprolactone). PEG of Hydrophilic block play an important role for protecting any degradation. Poly(lysine) was improved for cell permeability, and poly(caprolactone) was hydrophobic polymer as biodegradable, respectively. Moreover, disulfide bond was reducing by glutathione of intracellular so it was a key that stimuli-sensitive polymer. We removed empty particle or non-encapsulated plasmid DNA by Anion-exchange chromatography, so we observed transfection assay in HEK293 cell. |
