| 초록 |
This work reports the formation of spherical silica-encapsulated liposome particles (SLPs) as a function of the concentration of silica precursor, reaction time, temperature, and volume ratios of solvent, respectively. The solid SLPs have more robust and better loading-efficiency than those of liquid liposomes in carrier formulation. The liquid liposomes are hard to handle due to the easily fragile for the external stimuli and lower loading efficiency. The SLPs were obtained by the silicification with tetraethyl orthosilicate (TEOS) of the hydrophilic region of phospholipid bilayers by sol-gel process, and characterized by SEM, focused-ion beam(FIB)-SEM, confocal laser scanning microscopy (CLSM), TGA, Zeta potential, XRD, FT-IR, and UV-Vis. The obtained SLPs have a morphology of spherical shape with the average size of 2~3 μm, and the hydrophilic region of phospholipid bilayers in SLPs was confirmed by CLSM with green fluorescent FITC-labeling. The silica-encapsulation technique on the liquid liposomes gives lots of advantages such as a good particles-dispersity and stability. Furthermore, in vitro cytotoxicity assessment with the SLPs was achieved and showed the good cell viability about 82 %. |
