| 초록 |
We demonstrate a molecular net for capturing, release, and transportation of molecules by polymer-based DNA molecular net (PD-mn). Target biomolecules were captured from a mixture of biomolecules and released by a stimulus without degradation by PD-mn prepared by polymerization of acrylamide and Acryd-DNA. Furthermore, by introducing fluorescence-labeled competitor DNA, single-mismatched DNA was specifically detected. As a concept of artificial transporter, PD-mn transported target biomolecules from heterogeneous mixture of molecules into a specific enzymatic reaction solution and induced enzyme-mediated reaction. Finally, effective in vivo anti-tumor effect was demonstrated utilizing sheet-shaped PD-mn which has improved capture ability against VEGF as a target biomolecule by amplified DNA VEGF aptamer. This PD-mn could be used as not only an artificial transporter for specific capture and release of target molecules, but therapeutics by capturing of disease-related molecules. |
