| 초록 |
Bio-butanol has become another solution for the alternative to gasoline fuel. Its better characteristics of higher energy content, less hydroscopic and volatile than ethanol is regaining the reputation of conventional ABE (Acetone-Butanol-Ethanol) fermentation. However, the potent toxicity of butanol on the main ABE producers is still a major obstacle for economic production as a fuel. Extensive studies have been done only to reach the best titer around 20 g/L. In this study, we developed new HTS (High-Throughput-Screening) systems based on designed enzymatic reaction. Along with HTS system, we designed a programmed chemical mutagenesis system suitable to screen strains resistant to membrane toxicity. Using our original HTS system, we successfully developed a mutant strain, Clostridium beijerinckii CBP-381, which produced at least 30% more butanol. |
