| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Rapid and Highly Sensitive Detection of Enrofloxacin in Milk through LateralFlow Immunoassay Technique |
| 초록 |
Enrofloxacin(ENR) is an antibiotic endangering human health. For the rapid detection ofENR, in this work, we developed a one-step detection lateral flow immunochromatographicassay (LFIA) based on gold nanoparticles (AuNPs). An anti-enrofloxacinmonoclonal antibody (mAb) was conjugated with AuNPs for the specific detectionof enrofloxacin in milk samples. In the present study, the visual limit ofdetection (vLOD) was estimated to be 50 ng/ml which is the MRL allowed in milksamples by MFDS. The ENR test strip showed negligible cross-reactivity with ENRanalogues (ciprofloxacin, ofloxacin, norfloxacin), this indicates the highspecificity of the test strip toward ENR. The visual test results can be seenwithin 10 min without need of special equipment. |
| 저자 |
유진곤, Munirah Alhammadi, 허윤석
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| 소속 |
인하대 |
| 키워드 |
Biosensor; antibody; antibiotic; enrofloxacin; immunochromatographic assay; test strip; rapid test
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| E-Mail |
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