| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 봄 (04/04 ~ 04/06, 대전컨벤션센터) |
| 권호 |
43권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Protein-Polymer Nanocarrier based on Nitrilotriacetic Acid-End-Functionalized Poly(ε-caprolactone) for Drug Delivery System |
| 초록 |
we synthesized nitrilotriacetic acid-end-functionalized polycaprolactone (NTA-PCL) for the formation of degradable polymer nanocarrier conjugated with hexahistidine-tagged green fluorescence proteins (His6-GFP). NTA-PCL was prepared using initiators containing NTA moiety via ring-opening polymerization (ROP) and the structure of NTA-PCL was characterized using 1H NMR, and SEC. The GFP/PCL nanocarriers loaded with Nile Red were constructed by adding DMF solution containing nickel-complexed NTA-PCL (Ni-NTA-PCL) and Nile Red into phosphate buffer solution (PBS) containing His6-GFP. The diameter and size distribution of particles were analyzed by TEM and DLS. The conjugation with His6-GFP and encapsulation of Nile Red were analyzed by super-resolution confocal microscopy (SRCM) and PL spectroscopy. |
| 저자 |
황아란, 이채연, 백현종
|
| 소속 |
부산대 |
| 키워드 |
protein-polymer nanocarrier; polycaprolactone; drug delivery system
|
| E-Mail |
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