| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Tissue Engineering Disc using PLGA/DBP scaffolds |
| 초록 |
Since there is no optimal treatment for back pain associated with disc degeneration, focus has shifted toward replacement using tissue engineered disc. Demineralized bone particle was obtained bovine and has a powerful inducer of bone and cartilage specialization. We were fabricated natural/synthetic hybrid scaffolds using PLGA and DBP. Scaffold seeded on disc cell was cultured during 4weeks. Morphology of scaffolds and cell proliferation was characterized by SEM and MTT assay. Expressed gene such as aggrecan and type II collagen was confirmed RT-PCR. Influence of DBP content for tissue formation was investigated by H&E, Safranin-O, Masson’s trichrome staining. In SEM results, we observed that all scaffold has uniform porosity. But PLGA/DBP scaffold has good cell viability and attachment. In histology result, Scaffold containing DBP was the more the density of disc cell than PLGA only. Acknowledgements: This research was supported by KMOHW(0405-BO01-0204-0006) and SCRC(SC3100). |
| 저자 |
고연경1, 최방실1, 하현정1, 김순희1, 박기숙1, 김문석2, 이해방2, 이종문1, 강길선1
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| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
scaffold; DBP; tissue engineering; human disc cell
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| E-Mail |
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