| 초록 |
Some of Methylorubrum species have known as formatotroph, a portmanteau of formate and autotroph, however, those can utilize only small amount of formate as a sole carbon source. In addition, it has known that there is no enough information about related enzymes. In this study, we used Discovery Studio with CHARMm, molecular dynamics engine, for enzyme engineering. We hypothesized that the regulation of structure in enzyme level can improve substrate uptake. First, we can build the suitable homology model based on the known sequences and structures. Using constructed model, we are able to calculate CDOCKER Energy for estimating stability of enzyme when the enzyme binds to substrate. According to the serial results, we can find the appropriate candidates of mutation sites for enzyme stability. Finally, modified enzyme structure might show improvement of formate uptake. Based on the results, Discovery Studio has great potential as finding mutation points for enzyme engineering. |
