| 초록 |
Formate, the C1 substrate, is the simplest organic acid and reported as one of the desirable bulk chemical regarding economic and environmental benefits. However, lots of microorganism cannot use formate because of its toxicity and relating enzymes. In this study, we used Discovery Studio with CHARMm, molecular dynamics engine, for enzyme engineering. We hypothesized that the regulation of structure in enzyme level can improve substrate uptake. First, we can build the suitable homology model based on the known sequence and structure. Using constructed model, we are able to calculate CDOCKER Energy for estimating stability of enzyme when the enzyme binds to substrate. According to the serial results, we can find the appropriate candidates of mutation sites for enzyme stability. Finally, modified enzyme structure might be show improvement of formate uptake. Based on the results, we might get great mutant to improving formate uptake as a sole carbon source. |
