| 초록 |
Here we demonstrate recent technologies highlighting the cancer exosome detection and isolation simultaneously by introducing lectin and Janus nanoparticles. Lectins are glycoproteins that exhibit specific binding affinity for the saccharide moiety of glycoproteins. Cancer cells are known to express or secrete glycoproteins with specific saccharide chain like sialic acid in pancreatic cancer. Therefore we have been screening lectins that has affinity with sialic acid [e.g. Aleuria aurantia lectin (AAL), Sambucus nigra agglutinin (SNA), Pinellia ternata lectin (PTL)]. In this study, we test SNA lectin for capturing pancreatic cancer exosomes through bioconjugation with Janus nanoparticles that has two kinds of physiochemical characteristics. In the near future, we are going to separate exosome captured lectin-Janus nanoparticle using dielectricphoresis (DEP) system for the more efficient exosome isolation. |
