| 초록 |
Aspergillus niger is one of the major causes of human allergy and asthma in our daily lives. A need for rapid detection of such airborne fungus with capability of real time monitoring is continuously increasing, which will impact on human health and safety. To achieve this, specific binding ligands with high thermal and biochemical stabilities is strongly required. In this study, we identified specific binding peptides for the spore of Aspergillus niger by phage display screening as alternatives to antibodies. In flow cytometry assay, we confirmed that the identified peptide ligand has higher affinity toward the target spores than previously reported antifungal peptides. In addition, multivalent forms of the peptides were proven to have significantly higher affinity. Furthermore, those identified peptide ligands showed higher storage stability than antibodies. Finally, we studied the feasibility of fungal sensor systems based on the identified peptide ligands. |
