| 초록 |
Glucose level in whole blood is a clinical indicator of diabetes. For rapid and simple diagnosis of diabetes, many different types of point-of-care tests have been developed. In this study, we demonstrate a facile one-pot gelation-based bioassay via the quinone tanning of catechol-conjugated polyethylene glycol (PEG-C). We found that horseradish peroxidase (HRP) induces oxidation of catecholic precursors in the presence of hydrogen peroxide (H2O2). Therefore, a glucose-catalyzed one-step bioassay could be designed by coupling HRP with another enzyme, glucose oxidase (GOx), that can continuously produce H2O2 while metabolizing glucose. In our system, 10 mM of glucose was detected within 20 minutes. Compared to the conventional biomarker detection technologies, this system has the advantage of enabling fast and convenient detection of biomarkers without expensive equipment. In addition, the biomarker-triggered quinone tanning does not require any washing step to remove excess probes. |
