| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 봄 (04/08 ~ 04/09, 대전컨벤션센터) |
| 권호 |
35권 1호 |
| 발표분야 |
세포기능조절 고분자(의료용 고분자 부문위원회) |
| 제목 |
The Inflammatory Responses to PLGA Scaffold Depending on Silk Fibroin Conents: In Vitro and In Vivo Test |
| 초록 |
This study was designed to investigate the effect of silk fibroin as a natural material on the local inflammatory reaction of PLGA in vitro and in vivo. We manufactured five different ratios of PLGA/silk fibroin hybrid materials, with each material containing 0, 10, 20, 40 and 80 wt% of silk fibroin of PLGA by solvent casting/salt leaching method. To evaluate the influence of silk fibroin content in PLGA materials on cell viability, NIH/3T3 mouse fibroblasts were cultured in PLGA and the 5 different ratios of PLGA/silk fibroin scaffold. PLGA/silk fibroin films for the in vivo study were implanted in 4-week-old Wister rats. Cell proliferation activity was measured using MTT assay. ELISA for TNF-a was conducted to evaluate the inflammatory response of cells on the PLGA/silk fibroin scaffolds, the lowest figures showed in PLGA scaffold contained 80% silk fibroin. This research was supported by SCRC(SC4110) and WCU(R31-20029) |
| 저자 |
김수진, 고마음, 정선영, 김혜린, 송재은, 김세호, 이동원, 강길선
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| 소속 |
전북대 |
| 키워드 |
silk fibroin; inflammatory; PLGA scaffold
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| E-Mail |
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