| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
(생물공학) 생물산업의 미래를 위한 신기술: 분자로부터 시스템까지 |
| 제목 |
Adaptive laboratory evolution of a genome-reduced Escherichia coli |
| 초록 |
An iterative design-build-test-learn cycle was applied to genome-reduction in Escherichia coli. A genome-reduced strain harboring 3,457 genes in total was generated that showed impaired growth in minimal media that could not be understood based on the genes removed. We thus deployed adaptive laboratory evolution (ALE) to re-optimize growth performance of the genome-reduced strain. We showed that the genome-reduced strain served as a better host for the production of proteins of interest encoded by highly transcribing genes. Re-optimization using ALE reveals that failure of prediction may not be associated with understanding individual gene products but rather result from insufficient understanding of the genome-reduced strain’s systems biology. |
| 저자 |
조병관 |
| 소속 |
Korea Advanced Institute of Science and Technology |
| 키워드 |
Synthetic Biology; Minimal Genome; Adaptive Evolution
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| E-Mail |
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