| 학회 |
한국고분자학회 |
| 학술대회 |
2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 |
30권 1호, p.100 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Hepatocyte Adhesion on PVLA-coated Poly(lactic acid) Surface |
| 초록 |
Surface of poly(L-lactic acid) (PLLA) was modified by coating poly-[N-p-vinylbenzyl-4-O-b-D-galactopyranosyl-D-glucoamide] (PVLA), which was employed to improve the hepatocyte adhesion owing to its amphiphilic property and the presence of hepatocyte recognition part. We characterized the surface properties through water contact angle, electron spectroscopy for chemical analysis (ESCA) and scanning probe microscopy (SPM). The effect of PVLA coating on the efficiency of hepatocyte adhesion was evaluated by protein assay and optical microscopy. The surface morphology was under the influence of the concentration of PVLA coating solution and it played a critical role in hepatocyte adhesion. It was confirmed that galactose moieties in PVLA, which can bind to the asialoglycoprotein receptor (ASGPR) on hepatocyte, have more dominant effect on hepatocyte adhesion than enhanced hydrophilicity. We suggest that PVLA-PLLA system will be a useful method to improve hepatocyte cell seeding and adhesion onto scaffold matrix. |
| 저자 |
이재성1, 윤남균1, 김상헌2, Toshihiro Akaike3, 김성철1
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| 소속 |
1한국과학기술원, 2한국과학기술(연), 3Tokyo Institute of Technology |
| 키워드 |
PVLA; Hepatocyte; PLLA
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| E-Mail |
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