| 학회 |
한국고분자학회 |
| 학술대회 |
2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 |
28권 2호, p.72 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
In vitro non-viral mediated VEGF gene transfer into HUVEC for angiogenesis using low molecular weight chitosan/DNA nano complex |
| 초록 |
Depolymerized chitosan (DP-CS, molecular weights of 8517) was employed to investigate the DNA condensation ability as a gene carrier and a therapeutic agent for angiogenesis. DP-CS was chemically prepared with sodium nitrite in 6% acetic acid at 25℃. The molecular weight of DP-CS was determined by a gel permeation chromatography (GPC). ytotoxicity on HUVEC of DP-CS was measured by MTT assay. Scanning electron microscopy (SEM) was used to examine the morphology of the chitosan/DNA complex. Transfection efficacy and VEGF protein production were measured by β-galactosidase assay and VEGF protein kit, respectively. Angiogenesis influenced by DNA/DP-CS complex was measured by an optical microscopy as time went by. The cytotoxicity of DP-CS on HUVEC was little than that of the control by MTT. The size of DNA/DP-CS complex was within the range of 150 to 400nm. Transfection efficacy using VEGF DNA/DP-CS nano complex was 6 times than that using naked DNA. The maximum amount of VEGF protein produced by introducing DNA/DP-CS complex into HUVEC was about 580ng/ml. DP-CS is non-toxic and has good transfection efficacy. |
| 저자 |
방시원, 김중현
|
| 소속 |
연세대 |
| 키워드 |
nano-complex; non-viral; gene delivery; angiogenesis; nanoparticle
|
| E-Mail |
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