| 초록 |
Heparin-functionalized PLGA nanoparticles were prepared by a solvent-diffusion method for an efficient delivery of heparin-binding proteins. Their size distributions, surface charge and constitutional ratio of each components were evaluated. The entrapment of heparin molecules was confirmed by a negatively increased zeta potential value. Average diameter and surface charge of nanoparticles were changed by varying the amount of heparin, PLGA, and Pluronic. Constitutional ratio, evaluated by 1H NMR and anti-Xa heparin activity assay, revealed that the amount of heparin entrapped increased for a fixed mole ratio of PLGA and Pluronic as the amount of heparin increased during the preparation of nanoparticles. As a model in vitro release experiment, lysozyme was loaded into heparin-functionalized nanoparticles, and a sustained release profile was obtained. |
