| 초록 |
We develop a sensing assay of doxorubicin (DOX) by fabricating the fullerene (C60)-based molecularly imprinted polymer (C60MIP). An ARGET-ATRP initiator with an aldehyde groupis grafted onto fullerene by 1,3-dipolar cycloaddition reaction using N-methylglycine. The C60 initiator is used for the polymerization of methacrylic acid and ethylene glycol dimethacrylate in the presence of DOX. C60MIP is obtained by the removal of DOX from the polymer matrix. The fluorescence quenching of DOX by C60MIP in methanol is investigated. We observe the fluorescence of DOX (200 ng mL-1) is quenched gradually in the presence of C60MIP (10 mg mL-1), leading to an equilibrium value after approximately 12 h. When the same experiment is carried out using quinizarin, dantron, or rhein as an analyte, the amount of fluorescence quenching is much less. This result suggests that the fluorescence quenching occurs by electron transfer between DOX bound into the cavity and C60 in the polymer matrix. |
