| 초록 |
We compared the growth and ethanol production performance of Pichia stipitis KCCM 12009 in the presence of acetate, furans and phenolics in complex medium. We measured the adaptive response of P. stipitis to lignocellulosic hydrolysates. In the case of acetate, acetate 1 g/L showed the highest cell growth and ethanol production whereas the lowest level of cell growth and ethanol production were obtained for acetate 10 g/L. Furfural and 5-HMF were converted into furfuryl alcohol and HMF alcohol, respectively. These inhibitory compounds of furans did not significantly affect the final ethanol yield. Addition of 1 g/L of phenolics led to strong damage in the P. stipitis comparison to growth conditions in same concentration of acetate and furans. For all model compounds tested, the case of furfural 5 g/L and phenolics concentration over 3 g/L, P. stipitis did not grow up and it caused no ethanol production. Lignocellulosic hydrolysates from construction and demolition wood wastes (C&D) were also fermented and average value of ethanol yield (48 hr) was about 0.44. |
