| 초록 |
In this study, S. cerevisiae was engineered improve 1,2-propanediol production. Deletion of tpi1 (triosephosphateisomerase) gene in S. cerevisiae increased carbonflux to DHAP (dihydroxylacetonephosphate) in glycolysis, resulting in increased glycerol production. Then, mgs and gldA genes of which the products convert DHAP to 1,2-propanediol were introduced to the tpi1 deleted strain using a multicopy plasmid. As expected, the intracellular level of methylglyoxal was increased by introduction of mgs gene in S. cerevisiae and that of 1,2-propanediol by introduction of both mgs and gldA genes. As a result, 1.11 g/l of 1,2-propanediol was achieved in 168 flask culture. We overexpressed fps1p gene in S. cerevisiae for increasing extracellular concentration of 1,2-propanediol and achieved higher concentration of 1,2-propanediol in extracellular part of the culture. |
