| 학회 |
한국재료학회 |
| 학술대회 |
2014년 가을 (11/27 ~ 11/28, 대전컨벤션센터) |
| 권호 |
20권 2호 |
| 발표분야 |
D. 바이오 재료(Biomaterials) |
| 제목 |
Porous bone substitutes from avian eggshell biphasic calcium phosphate and eggshell membrane collagen I |
| 초록 |
Bone is an inorganic/organic hybrid consisting of apatitic calcium phosphate and organic (mostly collagen) constituents. Eggs on the other hand is has an eggshell which is a calcium rich source and an eggshell membrane (ESM) which is mostly made up of collagen. In this study, we developed a porous scaffold from eggshells and ESM. Biphasic calcium phosphate (BCP), with HAp/TCP ratio of 7:3, was synthesized using hen’s eggshell as calcium source and phosphoric acid by precipitation method. Collagen-I (Col-I) was extracted from the ESM by acid-pepsin digestion and isolated by differential salt precipitation. XRD analysis confirmed the synthesis of BCP and SDS-PAGE followed by FPLC confirmed the isolation and purity of col-I. Porous BCP scaffolds were fabricated using sponge replica method then coated with col-I. The fabricated scaffolds have an interconnected macroporous structure with high porosity and compressive strength. In vitro tests using MC3T3-E1 cells showed that the egg-derived scaffolds are osteoconductive and osteogenic. In vivo implantation in rabbit femoral head defects also showed the bone regenerative effects of the egg-derived bone substitutes. |
| 저자 |
Nathaniel T. Carpena1, Swapan Kumar Sarkar1, Celine DG Abueva2, Young-Ki Min3, Byong Taek Lee4
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| 소속 |
1InoBone Corporate R&D Center, 2Department of Regenerative Medicine, 3College of Medicine, 4SoonChunHyang Univ. |
| 키워드 |
Bone; Tissue Engineering; Calcium Phosphate; Eggshell; Eggshell Membrane
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| E-Mail |
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