| 초록 |
The bacterial flagella are driven by a flexible rotary nano-engine made up of protein. Its shape is generally a 20 nanometer-thick hollow tube. The development of direct detection methods of bacterial motion is a very important for their bio-motor applications. In addition, atomic force microscope (AFM) can serve as multifunctional, highly sensitive and potential biological detectors. Here we show a new technique for measuring the motion of a single bacterial flagellum by AFM with a modified cantilever. The reconstruction of AFM probe was carried out by FIB (Focused Ion Beam) procedure and hydrogel coating of the cantilever. Bacterial flagella motion and their behaviour were characterized by atomic force microscope, nanoindentation mode and scanning electron microscope. [This research was supported by Nano-Material Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning. (2014, 2009-0082580)] |
