| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 가을 (10/06 ~ 10/08, 제주 ICC) |
| 권호 |
39권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
A Phosphorylated EdU for an advanced DNA labeling agent |
| 초록 |
A novel thymidine analog, 5-ethynyl-2’-deoxyuridine (EdU), was developed as a DNA labeling agent. The alkyne group of EdU can be detected by fluorescent azide via “click chemistry”. The fluorescent azide can be easily diffused through tissues and can approach the incorporated EdU without DNA denaturation. However, the definite cytotoxicity of EdU has been reported that cells will eventually die after continuous incubation with EdU. We attempted to modify the EdU for reduced cytotoxicity and efficient incorporation into DNA. EdU which contains alkyne group for the “click chemistry” with fluorescent azide was synthesized from IdU. Hydroxyl group of phosphate was masked by bis-pivaloyloxymethyl (POM) protecting group to enhance cell membrane permeability. And bis-pivaloyloxymethyl phosphate was added to EdU. Its biological activities were compared to commercialized EdU in vitro. |
| 저자 |
서시웅1, Kazumitsu Onizuka2, Hiroshi Abe1, Satoshi Tsuneda3, Yoshihiro Ito1
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| 소속 |
1Nano Medical Engineering Laboratory, 2RIKEN., 3RIKEN |
| 키워드 |
EdU; Phosphorylation; DNA labeling; Cytotoxicity
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| E-Mail |
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