| 초록 |
Chitosan, the second most abundant polysaccharide, has been studied as biomedical material due to its biodegradability and biocompatibility. It is widely used as a wound healing dressing because it has a hemostatic potential. Herein we reported that phosphorylation of chitosan improved its hemostatic ability preserving the biocompatibility. Phosphorylated chitosan synthesized with a phosphorus pentoxide/ methanesulfonic acid system was confirmed by XRD, FT-IR, 31P NMR, and 13 C NMR. We tested the antibleeding properties of the modified chitosans with various ratio of phosphate substitution by applying them on a mouse hemorrhage liver. As a result, the modified chitosan with higher degree of phosphate reduced a blood coagulation time. The phosphorylation resulted in the porous and anionic surface of chitosan, which had important role in assembling proteins responsible for the blood coagulation initiation. It indicated that phosphorylation accelerated the blood clot formation. |
