| 초록 |
Control of cell-matrix adhesion has become the most important issue in the regulation of stem cell function. In this study, a basic fibroblast growth factor (FGF2)-immobilized substrate (FGF2 substrate) was applied as an artificial matrix to regulate integrin-mediated signaling. The phosphorylation of focal adhesion kinase, cytoskeletal re-organization, and cell proliferation were restricted in human mesenchymal stem cells (hMSCs) adhering to the FGF2 substrate compared to a fibronectin-coated substrate (FN substrate). MS analysis revealed that 2,3-sialylated glycans, a potential marker of stem cell function, were more abundant on hMSCs expanded on the FN substrate than on those expanded on the FGF2 substrate. Thus, the “stemness” of hMSCs is controlled by the type of adhesion substrate. Elucidation of the glycan structure on the cell membrane may help characterize hMSC function. |
