| 초록 |
The unique flexibility and throughput of cell-free protein synthesis have made this technology a promising alternative to conventional cell-based protein production methods. However, the limitations associated with the cost and utilization efficiency of energy sources have remained a major issue to further improve cell-free protein synthesis. Although glucose can be used as an energy source without phosphate accumulation, the use of glucose has the problem of proton accumulation, which inhibits the efficiency of protein synthesis. In this study, we improved the efficiency of ATP regeneration from glucose through secondary utilization of formate generated from glucose. In E.coli, formate lyase converts pyruvate produced from glucose into acetyl-CoA and formate. We attempted to use formate for additional regeneration of NADH in the presence of recombinant NAD-dependent formate dehydrogenase and expected that the regenerated NADH would be used for regeneration of ATP through the oxidative phosphorylation reaction. |
